Genomic analysis of circulating HIV and hepatitis C virus infections and coinfections in Cameroon: 2005–2006 and 2015–2016

Introduction In humans, RNA viruses are responsible for a wide range of acute, chronic, emerging and re-emerging infections. Human Immunodeficiency virus (HIV) hepatitis C (HCV) rank as some the most important public health challenges affecting Africa. Methods We performed enzyme-linked immune-sorbent assays to confirm positive specimens, genomic characterization on two cohorts people living with HIV in Douala Yaoundé periods 2005-2006 2015-2016. These groups were tested co-infection HCV using immunosorbent assays. Viral was extracted from patients’ plasma samples by QIAGEN method, specific primers used amplify genes interest genomes. The amplification products subsequently cloned sequenced. nucleotide sequences aligned, genotyped phylogenetically analyzed. Results isolate identified this study belongs HIV-1 group M Subtype A1. subtypes characterized 1h 4t corresponding dominant strains that circulate Cameroon. Phylogenetic analysis NS5B gene showed cluster Gabonese, Canadian, previously sequenced Conclusion perspectives results shed light genetic diversity Virulent infections common Cameroon, therefore there is great need further viral evolutionary spatio-temporal patterns.